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Overseas Field Training：Investigation of the bacteria limiting green house gases emission in the rice paddy in Thailand
Investigation of the bacteria limiting green house gases emission in the rice paddy in Thailand
Miu Kamimura
Destination and period: Thailand, August 27 - September 4, 2011
In recent years, greenhouse gases emission from agricultural field is one of the serious environmental problems around
the world. In addition, the rice paddy is the main source of methane, one of the greenhouse gases. Thailand has rice
growing culture. The crop area in Thailand is 10.94 million ha. And Thailand has a No.1 share of quantity of export in
the world in 2009. It is said that Rice cultivation in Thailand has the big impact for the environmental problem. For the
approach against the problem, knowledge about process of producing or using green house gases by microorganisms
in the rice paddy is necessary.
With considering this situation, the bacterial status and environmental status of rice paddy, such as surface water quality, are
especially studied in this oversea training. On this training, I focused on the bacteria that used methane and not produce nitrous
oxide in the reaction process. And I would like to establish a causal connection between bacteria and environment status.
Soil
and surface water samples were collected from the 3 rice paddy fields. One sampling point is directly connected to the Chao
Praya River, and its wastewater flow into the river (sample 1). The other sampling point is in the industrial area (sample 2). And
last sampling point is in the Kasetsart University (Kamphaeng Sean Campus) site (sample 3). But water sample was not collected
in sampling point 3 because of lack of preparation. DNAs of all bacteria were extracted from the rice paddy soil. (Extracted DNA PCR
analysis) Unfortunately, the bacterial class could not be identified from the samples of rice paddy connected to the Chao Praya River
and in the industrial area. The result suggested that the bacteria are minority in these rice paddies another sample will be analyzed.
Key words: Rice paddy field, Greenhouse gases, Chao Praya River, Bacteria