僀僱偄傕偪昦嬠傪庛撆壔偡傞儅僀僐僂僀儖僗偺慡墫婎攝楍偲偦偺僂僀儖僗棻巕
Molecular characterization of Magnaporthe oryzae chrysovirus 1 (MoCV1) that attenuates host rice blast fungus and purification of the virus particles
壛摗岾塰丒仠楅栘 桟丒塝嶳弐堦丒惵栘嵷乆巕丒桳峕 椡帥壀 揙丒暉尨晀峴丒怷嶳桾廩
Kato, S., Suzuki, Y., Urayama, S., Aoki, N., Arie, T., Teraoka, T., Fukuhara, T., Moriyama, H

Abstract

僀僱偄傕偪昦嬠偵姶愼偡傞MoCV1乮M. oryzae chrysovirus 1乯偼, 係偮偺暘愡僎僲儉dsRNA傪桳偟, 廻庡嬠偺惗堢傗暘惗巕宍惉偺慾奞偲偄偭偨庛撆壔傪堷偒婲偙偡. 偙傟傑偱偺晹暘揑側墫婎攝楍夝愅偵傛傝, dsRNA 1 偼RdRP_4曐懚儌僠乕僼傪桳偡RNA埶懚RNA暋惢峺慺傪僐乕僪偟, chrysovirus壢偵懏偡傞偙偲偑帵嵈偝傟偨乮壛摗傜丆楅栘傜丆暯惉20擭搙搰崻戝夛乯. 崱夞, MoCV1偺係偮偺dsRNA偺慡墫婎攝楍傪柧傜偐偵偟偨. 偙偺MoCV1偑塼懱攟梴忋惔拞偐傜専弌偝傟傞偙偲傪慜戝夛偱弎傋偨偑, 崱夞偙偺忋惔拞偐傜僂僀儖僗棻巕偺惛惢傪帋傒偨偲偙傠, 僄儞儀儘乕僾條偵曪傑傟偨捈宎栺30nm偺惓懡柺懱峔憿偺棻巕偑娤嶡偝傟偨. 偙偺僂僀儖僗夋暘傪SDS-PAGE偱夝愅偡傞偲栺70kDa偺僞儞僷僋幙偑専弌偝傟偨. 尰嵼, 梊憐偝傟傞奜旐僞儞僷僋幙偺攝楍忣曬傪尦偵儁僾僠僪峈懱傪嶌惢偟, 攟梴忋惔拞偵懚嵼偡傞僂僀儖僗擹搙傗僂僀儖僗愙庬幚尡傪専摙拞偱偁傞.

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