Abstract

搚忞拞偵愽嵼偡傞昦尨嬠偺摿堎専弌偼丄搚忞昦奞杊彍懳嶔忋廳梫偱偁傞丅rDNA-IGS椞堟偺PCR-DGGE偍傛傃億儕僈儔僋僣儘僫乕僛堚揱巕忋偵愝寁偟偨摿堎僾儔僀儅乕乮Hirano & Arie, 2007乯傪梡偄偨PCR偵傛偭偰丄乽懡條側搚忞旝惗暔乿亜乽Fusarium oxysporum乿亜乽僩儅僩堔挏昦嬠乮F. oxysporum f. sp. lycopersici乯乿亜乽昦尨惈儗乕僗乿偲丄戝暘椶孮偐傜彫暘椶孮傊弴師偵饪偄暘偗偡傞偙偲偱幆暿偑壜擻偱偁傞偙偲傪婛偵曬崘偟偨乮媑壀傜 2008丟惷壀戝夛乯丅杮朄偼搚忞拪弌DNA乮eDNA乯傪拻宆偲偟偨応崌傕桳岠側傕偺偺丄暋悢儗乕僗偑嫟懚偡傞応崌偵偼幆暿偑崲擄偱偁傞摍丄幚梡忋偺栤戣揰偑巆偝傟偰偄偨丅偦偙偱杮尋媶偱偼丄Taqman僾儘乕僽傪梡偄偨儕傾儖僞僀儉PCR偵傛傞摿堎幆暿朄偺妋棫傪帋傒偨丅(1)rDNA-IGS椞堟偺墫婎攝楍偑擔杮嶻僩儅僩堔挏昦嬠偱偼儗乕僗乮1乣3乯枅偵堎側傞偙偲乮Kawabe et al. 2005丟堫尒傜 2008丟 惷壀戝夛乯偵婎偯偒丄摨椞堟撪偺墫婎攝楍懡宆傪擣幆偡傞僾儔僀儅乕乛僾儘乕僽僙僢僩sp1-2f+sp1-2r乛sp1-2pr乮儗乕僗1幆暿梡乯丄sp2-2f+sp2-2r乛sp2-2pr乮儗乕僗2幆暿梡乯丄sp3f+sp3r乛sp3pr乮儗乕僗3幆暿梡乯丄(2)僩儅僩堔挏昦嬠偑摿堎揑偵曐帩偡傞昦尨惈娭楢堚揱巕椞堟忋偺SIX1堚揱巕乮van der Does et al. 2008乯傪擣幆偡傞僾儔僀儅乕乛僾儘乕僽僙僢僩SIX1f+SIX1r乛SIX1pr丄(3)僩儅僩堔挏昦嬠儗乕僗1偵摿桳側SIX4堚揱巕乮=AVR1丟Houterman et al. 2008乯傪擣幆偡傞僾儔僀儅乕乛僾儘乕僽僙僢僩SIX4f+SIX4r乛SIX4pr傪嶌惉偟偨丅F. oxysporum嬠懱偐傜拪弌偟偨DNA傪拻宆偲偟偰丄(1)偵傛偭偰僩儅僩堔挏昦嬠傪丄(3)偵傛偭偰堔挏昦嬠偺偆偪儗乕僗侾傪丄(1)偵傛偭偰擔杮嶻僩儅僩堔挏昦嬠偺奺儗乕僗乮1乣3乯傪摿堎揑偵幆暿偱偒偨丅偝傜偵丄偙傟傜偺僾儔僀儅乕乛僾儘乕僽僙僢僩傪梡偄偨儕傾儖僞僀儉PCR偑搚忞拞偺僩儅僩堔挏昦嬠偺幆暿傗掕検偵傕桳岠偱偁傞壜擻惈丄偙傟傜僾儘乕僽偺儅僀僋儘傾儗僀傊偺搵嵹偺壜擻惈偑帵嵈偝傟偨丅

*擾娐尋