Gibberella sacchari宍幙揮姺懱岎攝屻戙偵惗偠傞僴僀僌儘儅僀僔儞B懴惈堚揱巕偺曄堎偵偮偄偰
Mutations in hygromycin B resistance gene (hph) found in sexual cross of Gibberella sacchari-transformants
攇揷栰枹桼棃帥壀丂揙桳峕丂椡
Miyuki HatanoTohru TeraokaTsutomu Arie

Abstract

Gibberella sacchari乮anamorph: Fusarium sacchari乯偼堎側傞岎攝宆傪帩偮嬠姅娫偱岎攝偟姰慡悽戙傪宍惉偡傞僿僥儘僞儕僢僋乮self-incompatible乯側巕偺偆嬠偱偁傞丅FGSC 7610姅乮MAT1-2乯偲7611姅乮MAT1-1乯傪丆偦傟偧傟僾儔僗儈僪pMK412乮egfp hph; Watanabe et al., 2007乯偲pAK2-HYG乮dsred2 hph; 壛摗, 2008乯偱宍幙揮姺偟偨丅摼傜傟偨宍幙揮姺懱FGSC 7610-gfp-hph偲FGSC 7611-rfp-hph傪岎攝偟偰摼傜傟偨巕偺偆朎巕傪扨朎巕暘棧偟偨偲偙傠丆椢乮egfp - 乯丗愒乮- dsred2乯丗墿乮egfp dsred2乯丗敀乮- -乯偵暘棧偟偨丅椢丆愒丆墿偺寀岝傪帵偡扨朎巕暘棧姅偼棟榑忋僴僀僌儘儅僀僔儞B懴惈堚揱巕乮hph乯傪曐帩偡傞偼偢偱丆PCR偵傛偭偰hph堚揱巕偼妋擣偱偒偨丅偟偐偟丆偦偺拞偵僴僀僌儘儅僀僔儞B姶庴惈傪帵偡嬠姅偑擣傔傜傟丆堚揱巕宆偲昞尰宆偑柕弬偟偨丅偙傟傜柕弬偟偨嬠姅偺hph堚揱巕偺晹暘墫婎攝楍乮栺900 bp乯傪夝愅偟偨偲偙傠丆C:G偺2.5乣11.6亾偑T:A偵抲姺偟偰偄偨丅偙傟偼Neurospora crassa偵偍偄偰妋擣偝傟偰偄傞RIP乮repeat-induced point mutation; Selker et al., 1987乯偵椶帡偺尰徾偲峫偊傜傟偨丅


戞侾侽夞巺忬嬠暘巕惗暔妛僐儞僼傽儗儞僗乮俀侽侾侽擭侾侾寧侾俉乣侾俋擔丄搶峀搰巗乯億僗僞乕敪昞