僩儔儞僗億僝儞憓擖偵傛傞僩儅僩堔挏昦嬠偺旕昦尨椡堚揱巕偺婡擻憆幐
The loss of function of the avirulence gene AVR1 in the tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici by transposon-insertion
堫尒孿屽丒怷揷懽彶*丒帥壀丂揙丒桳峕丂椡
Keigo Inami丆Yasuaki Morita, Tohru Teraoka丆Tsutomu Arie

Abstract

怉暔昦尨嬠偱偁傞僩儅僩堔挏昦嬠Fusarium oxysporum f. sp. lycopersici偼僩儅僩昳庬偵懳偟偰昦尨椡傪堎偵偡傞3偮偺乽儗乕僗乿偵暘壔偟偰偄傞丅僩儅僩昳庬偲堔挏昦嬠儗乕僗偺恊榓惈乛旕恊榓惈偼丆奺昳庬偑桳偡傞堔挏昦掞峈惈堚揱巕偲奺儗乕僗偑曐帩偡傞旕昦尨椡堚揱巕AVR偺憡屳娭學偱寛掕偝傟傞丅椺偊偽丆儗乕僗1偼AVR1傪帩偪丆AVR1傪擣幆偡傞掞峈惈堚揱巕I傪曐帩偡傞僩儅僩昳庬偼儗乕僗1偵掞峈惈偱偁傞偨傔丆旕恊榓惈偺娭學偱偁傞丅儗乕僗1偑AVR1傪幐偆偲丆I偵傛傞掞峈惈偑柍岠壔偡傞偺偱丆恊榓惈偺娭學偵側傞丅偙偆偟偰怴偨側儗乕僗偑弌尰偡傞丅偮傑傝丆AVR1傪曐帩偟偰偄傞偺偼儗乕僗1偺傒偱偁傞乮Houterman et al. 2008乯丅2008擭廐偵崅抦導擔崅懞偱儗乕僗1掞峈惈昳庬偵敪惗偟偨堔挏昦偺暘棧姅KoChi-1偺僎僲儉傪拻宆偵丆AVR1摿堎僾儔僀儅乕傪梡偄偰PCR傪峴偭偨偲偙傠憹暆偑尒傜傟偨丅偟偐偟偦偺抐曅乮栺1500 bp乯偼儗乕僗1偐傜摼傜傟傞抐曅乮栺700 bp乯傛傝傕戝偒偐偭偨丅KoChi-1偐傜摼傜傟偨抐曅偺撪晹偵偼759 bp偺僩儔儞僗億僝儞偑憓擖偝傟丆AVR1偺ORF偑攋夡偝傟偰偄偨丅杮僩儔儞僗億僝儞偼hAT僼傽儈儕乕偵懏偟丆僩儔儞僗億僓乕僛傪僐乕僪偟側偄旕帺棩惈偺傕偺偱偁偭偨丅傑偨丆Broad Institute偺僎僲儉僨乕僞儀乕僗偵傛傞偲丆F. oxysporum偺僎僲儉拞偵摨條偺僩儔儞僗億僝儞偑70僐僺乕埲忋嶶嵼偟偰偄偨丅KoChi-1偺僎僲儉偵儗乕僗1桼棃偺AVR1傪摫擖偟偨宍幙揮姺姅偼丆AVR1偵懳墳偡傞掞峈惈堚揱巕I偺傒傪曐帩偡傞僩儅僩昳庬偵堔挏昦傪堷偒婲偙偣側偔側偭偨丅廬偭偰丆KoChi-1偼僩儔儞僗億僝儞偺憓擖偺偨傔偵AVR1偲偟偰偺婡擻傪憆幐偟偰偄傞偙偲偑柧傜偐偵側偭偨丅偙偺條偵僩儔儞僗億僝儞偑憓擖偝傟偨AVR1椞堟偼挷傋偨尷傝偺僩儅僩堔挏昦嬠偵偼傒傜傟偢丆昦尨惈恑壔偺堦偮偺帇揰傪帵偡傕偺偱偁傞丅

*崅抦導擾椦媄弍僙儞僞乕

戞俋夞巺忬嬠暘巕惗暔妛僐儞僼傽儗儞僗乮俀侽侽俋擭侾侾寧侾俉乣侾俋擔丄暥嫗嬫乯億僗僞乕敪昞
仏桪廏億僗僞乕敪昞徿庴徿仏