Isolation of live bacteria from environmental samples is considered as a challenging task in applied microbiology. In this project, we try to develop approaches than can be used for the direct isolation, cultivation and manipulation of a target bacteria.
Hard work in progress. We hope to share our achievements soon.
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RESEARCH HIGHLIGHTS / 研究成果
Abiotic Factors Promote Cell Penetrating Peptide Permeability in Enterobacteriaceae Models
PUBLICATION: Toyohara D et al.,Front Microbiol. 10:2534 (2019)
COLLABORATORS: Muraoka Lab, Tokyo University of Agriculture and Technology
SUMMARY: To evaluate the applicability of cell-penetrating peptides (CPPs) as a biomolecule carrier with diverse bacterial strains, we evaluated and optimized factors that may play an important role in elevating CPP permeation efficiency. Based on our evaluation, we showed that 2 abiotic factors, temperature and tonicity promoted CPP permeation. Using our optimized conditions, we showed that CPP permeation was promoted in 9 out of 10 Enterobacteriaceae strains.
